青霉素G(BP)ELISA快速檢測試劑盒
一、原理
本試劑盒采用間接競爭ELISA方法,在微孔條上預包被偶聯(lián)抗原,樣本中的殘留物青霉素將和微孔條上預包被的偶聯(lián)抗原競爭抗青霉素抗體,加入酶標記物后,用TMB底物顯色,樣本吸光值與其所含殘留物青霉素的含量成負相關,與標準曲線比較即可得出相應殘留物青霉素的含量。
二、試劑盒特性
u 試劑盒靈敏度: 0.1ppb
u 孵育溫度: 25℃
u 孵育時間: 30min~30min~15min
u 批內、批間差: 不超過10%
u 樣本檢測下限
組織 ················································ 2ppb
u 交叉反應率
青霉素· ········································ 100%
氨芐青霉素········································ 0.8%
鄰氯青霉素 ······································· 0.2%
雙氯青霉素 ······································· 0.1%
阿莫西林 ········································· 0.1%
頭孢塞呋 ········································· 0.1%
u 樣本回收率
組 織 ········································· 60%-120%
三、試劑盒組成
1 | 微量測試孔 | 每條8孔,一板12條 | |
2 | 標準液×6瓶 (1ml/瓶) | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | 酶標記物 | 12ml | 紅色帽 |
4 | 抗體工作液 | 7ml | 藍色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 終止液 | 7ml | 黃色帽 |
8 | 20X濃縮洗滌液 | 40ml | 白色帽 |
9 | 10X濃縮復溶液 | 50ml | 透明帽 |
四、樣品前處理步驟及檢測分析步驟
詳見產品說明書。
五、儲藏條件和保質期
1、 儲藏條件:試劑盒于2-8℃保存,不要冷凍。
2、 保 質 期:該產品有效期為1年,生產日期見包裝盒。
實驗代做服務:
| |||
|
| ||
| |||
|